Part:BBa_K4180000:Design

SNF1 is the metabolism sensor

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 528
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

For this SNF1 gene cloning, two restriction enzymes are used, XmaI and KpnI. For double digestion, sometimes it's hard to put two enzymes together at the same time, so sequential digestion is needed

Source

BY4741 YEAST STRAIN